Crispr Lab Take Two: What's Next?

First me, my brother,and my dad started by making the plates. But there was one little problem we didn't have ampicillin! When I checked them a couple weeks later some were contaminated. I could tell because there was this funky stuff growing on them (bacteria).

Next hydrating bacteria, everything was going well until we had the heat and cold shock .We are supposed to do the heat and cold shock for 30 minutes but we left for our grandparents and house and ended up doing the next step 4 hours later.

These are the tubes in the cold shock fase.

After that we added the CaCI2 to the mix but sadly we didn't have any CaCI2 so my dad went out for a run and he found some rock salt that we used instead of CaCI2. We were very happy that he had actually found some so we could keep going with our mission to finish the Crispr lab.

Time for the last step, everything went well besides the fact that we didn't have the soc recovery media! A week later we checked on the plates and sadly only two of them got 1 spot

of ecoli.

conclusion